Fig. 6

Extracellular vesicular Wnt7b mediates HPV E6-induced CC tumor progression through β-catenin signaling in vivo. a Representative images of tumor progression in mice from 4 groups (the mice were injected with PBS, EV/NC, EV/E6-KD or EV/E6-KD + Wnt7b-OE) were shown. b The final tumor volume of the mice in the 4 groups (PBS, EV/NC, EV/E6-KD and EV/E6-KD + Wnt7b-OE) was determined after the mice were euthanized. c In vivo angiogenesis was assessed by CD31 immunostaining. Haematoxylin and eosin-stained images and immunohistochemistry analysis of CD31 protein levels in xenograft tumor tissues were shown (bar: 100 μm). The results showed that the density of blood vessels (assessed via CD31 staining) was decreased in the EV/E6-KD tumours compared with the EV/NC tumours, while Wnt7b-OE abrogated the inhibition of tumor angiogenesis by EV/E6-KD. d Representative images of tumor progression in mice from 3 groups (the mice were injected with EV/E6-KD, EV/E6-KD + Wnt7b-OE, or EV/E6-KD + Wnt7b-OE+ FH535) were shown. e The final tumor volume of the mice in the 3 groups (EV/E6-KD, EV/E6-KD + Wnt7b-OE, and EV/E6-KD + Wnt7b-OE+ FH535) was determined