Fig. 4

Exosomal miR-7e-5p inhibits macrophage apoptosis by targeting FASL. a Target genes of miR-7e-5p were predicted using miRTarBase, miRDB and TargetScan. Intersections of the results were used for functional analysis using the Panther and GCBI online databases. b Western blotting detecting PARP, cleaved PARP and FASL levels in macrophages is shown. The macrophages in each well were cocultured with miR-7e-5p-mimics or inhibitors-treated A20 cells. Actin served as a control. Macrophages were pretreated with aclarubicin at 2.67 μg/mL. c Real-time PCR analysis of FasL expression in macrophages. To each well of macrophages, 1.2 × 10⁷ exosomes from miR-7e-5p-mimics (30 nM, 50 nM, 100 nM) or inhibitors (50 nM, 75 nM, 100 nM)-treated A20 cells were added. Statistical test: t-test