Fig. 5

Treatment with exosomes influences the apoptosis of macrophages. a Western blotting detecting PARP, cleaved PARP, Caspase-3, cleaved Caspase-3 and FASL in macrophages treated with exosomes from A20 cells transfected with miR-7e-5p-mimics (50 nM). GW4869 (10 μM) was added to A20 cells for 24 h to inhibit exosome secretion. b Flow cytometry was used to detect apoptotic macrophages by FITC-Annexin V and propidium iodide (PI) double staining. F4/80 was used for the identification of macrophages. Bar charts indicating the change of cell populations undergoing early apoptosis according to three independent experiments. Statistic test: t-test. c Representative images of TUNEL assays to assess macrophage apoptosis. Macrophages were pretreated with aclarubicin at 2.67 μg/mL for 2 h before adding exosomes. Macrophages were incubated with terminal deoxynucleotidyl transferase (TdT) for 2 h before detection. Scale bars: 30 μm