Fig. 2

Knock down of UPK1A-AS1 inhibits proliferation in HCC cells. a-b. CCK-8 assay was performed to determine the effect of si-UPK1A-AS1 on proliferation in SK-Hep-1 (b) and MHCC-97H (c) cells (^***P < 0.001). c-d. Proliferation curve of HCC cells transfected with sh-UPK1A-AS1 (^*P < 0.05, ^***P < 0.001). e-f. Representative CCK-8 analysis of cell viability after downregulation of UPK1A-AS1 by LNAs in SK-Hep-1(e) and MHCC-97H (f) cells (^*P < 0.05, ^**P < 0.01, ^***P < 0.001). g-j. EdU assay showed that downregulation of UPK1A-AS1 decreased the ratio of S phase cells in SK-hep-1(g-h) and MHCC-97H (i-j) cells (^*P < 0.05)