Fig. 1

LCL161 triggers lung cancer cell elongation, invasion and migration at a nontoxic concentration. a A549 and H1299 cells were treated with the indicated concentration of LCL161 or DMSO for 24 h. Cell viability was detected by the MTT assay. b H1299 / A549 cells were treated with 10 μM / 25 μM LCL161 or DMSO for 24 h. Cell morphology was analysed by phase-contrast microscopy. The cell elongation index (length/width) was determined by measuring cell length and width. Magnification, × 100. *P < 0.05. c, d A549 and H1299 lung cancer cells were plated and allowed to grow to confluence. The medium was removed, and wounds in the confluent monolayers were produced with a 100-μl pipette tip. After treatment with the indicated concentrations of LCL161 or DMSO for 24 h or 48 h, the wound-healing process was monitored by an inverted light microscope. e A549 and H1299 lung cancer cells were treated with the indicated concentrations of LCL161 or DMSO for 48 h. The migrated cells in a collagen-coated transwell migration chamber were fixed and stained with crystal violet, and optical density was measured. Magnification, × 100. Representative results are shown. Plots are the mean ± SEM of data from three independent experiments. *P < 0.05