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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma

Fig. 2

KMT5A is a direct target of miR-340-5p. a Intersection of miR-340-5p predicted targets from 4 prediction algorithms (PITA, miRanda, TargetScan, and microT), and 489 genes were selected. b Of the 489 genes, KMT5A was the only gene presented in both bioinformatics-predicted targets and miR-340-5p coexpression analysis. c-d KMT5A expression regulated by miR-340-5p was confirmed by RT-PCR (c) and western blotting (d). e The putative miR-340-5p-binding sites within the 3′ UTR of KMT5A mRNA. Relevant mutations are shown. f Reporter plasmids containing either the wild-type (wt) or mutated (mut) 3′ UTR of KMT5A were cotransfected with miR-340-5p. Luciferase activity was significantly decreased in wt, while mut remained unchanged. Asterisks (* and **) indicate a significant difference. *, P < 0.05; **, P < 0.01

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