Fig. 4

The miR-340-5p/KMT5A axis regulates CD8⁺ T cells. a Representative IHC images of the association between KMT5A expression and CD8⁺ T-TILs. Magnification of the microscope: left column, 40×; right column, 200×. b KMT5A overexpression was confirmed by western blotting. c Representative example and cumulative data of the virus-specific CD8⁺ T-cell functional avidity. d Each graph represents cytotoxicity curves for one DLBCL patient. CD8⁺ T-cell cytotoxicity was quantified with KMT5A-overexpressing or negative control LY-1 cells. Cumulative data of the fold change in cytotoxicity are shown. e KMT5A overexpression significantly reversed the miR-340-5p-induced increase in cytokine production in cocultured CD8⁺ T cells. Asterisk (*) indicates a significant difference. *, P < 0.05