Fig. 3

Triple positive Caspase-4, K-Ras and c-Myc NSCLC patients have poor survival rate. NSCLC patients were stratified according to the levels of caspase-4 related to PD-L1 expression (a), gene mutation (i.e. EGFR mutation, ALK or MET or ROS1 translocation) (b), or c-Myc overexpression (g, m) and K-Ras mutations (j, m). In addition, fraction of total was analysed (b, e, h, k, n) to better highlight the fraction of analysed patients according to the levels of tumor tissue caspase-4 based on a cut-off (377 pg/ml) obtained by ROC analysis. Furthermore, the percentage of caspase-4 positive patients were related to PD-L1 expression (c), gene mutation (i.e. EGFR mutation, ALK or MET or ROS1 translocation) (f), or c-Myc overexpression (i, o) and K-Ras mutations (l, o). Log-rank Mantel-Cox and Wilcoxon test were performed to statistically analyse the survival rate between the groups