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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Identification of a novel subpopulation of Caspase-4 positive non-small cell lung Cancer patients

Fig. 4

Caspase-11 is involved in tumor progression in mice. a Mouse model of NMU-induced carcinogenesis. b Wild type mice (n = 23) showed higher tumor lesions than caspase-11 ko (n = 12) mice in a time-dependent manner. c C3H mice (n = 14) did not show any difference in terms of tumor areas than wild type mice. d Lung tissues of K-Ras LA1 (n = 10) and K-Ras LA1p53R172HB (n = 10) had similar levels of cleaved caspase-11 as wild type NMU-treated C57Bl/6 mice. e BAL levels of IL-1β in caspase-11 ko mice (n = 12) were solely reduced at longer time points compared to wild type mice (n = 23); instead, f IL-1α levels in lung homogenates were significantly reduced at all time points in caspase-11 ko mice (n = 12) compared to wild type mice (n = 23) subjected to NMU. g The neutralization of IL-1α by means of a monoclonal antibody significantly reduced tumor hyperplastic cells in the lung of NMU-treated C57BL/6 mice. Lung immune infiltration was characterized by MDSC (h), but not by Treg (i) in NMU-treated wild type but not in caspase-11 ko mice. j No differences in tumor area was observed in NMU-treated mice or in mice who were subjected to the adoptive transfer (n = 10) of CD4+ T cells obtained by C57Bl/6 or caspase-11 ko mice. k Bone marrow transplantation experiments showed that wt into ko mice had similar tumor area as caspase-11 ko mice treated with NMU (n = 12). Data are expressed as mean ± SEM or as median ± quartile range (violin plots). Two-Way ANOVA followed by Sidak’s multiple comparison test was applied to Fig. B, C, E, F, H, I. The statistical analysis performed for Fig. B and C considered the comparison among and within the groups according to two variables, tumor lesion vs time; instead the statistical analysis for Fig. E and F was performed according to the variation of the amount of cytokine released (expressed as pg/ml) vs time, as well as in Fig. H and I the variables were percentage of cells vs time. In contrast, One Way ANOVA followed by Bonferroni’s post test was applied to Fig. G, J, K, as the statistical test was related to the variation within and among the groups according the only one variable

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