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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Identification of a novel subpopulation of Caspase-4 positive non-small cell lung Cancer patients

Fig. 5

Large subunit of caspase-4 facilitates tumor cell proliferation. A549 cells were transfected with PC4–1, PC4–2, PC4–3 and PC4–4 as explained in Materials and Method section. a Cells transfected with PC4–4 (the sole large subunit of caspase-4) showed higher CFSE positive cells than the other groups (n = 15). b Co-culture of A549 cells and PBMCs (ratio 1:5) showed similar proliferation expressed as %CFSE+ cells than A549 alone treated with the recombinant large subunit of capase-4. The neutralization of EGFR by means of cetuximab (c), inhibition of HDAC by means of SAHA (d), inhibition of methyltransferase (e) and mTOR (g) did not alter cell proliferation as instead observed when the inhibitor of k-RAS (FTI-276) was added (f). Experiments were performed n = 15 in duplicate. Data are expressed as median ± quartile range and represented as violin plots. One-Way ANOVA followed by Bonferroni’s post test was applied

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