Fig. 1

MCP-1 was involved in and promoted osteosarcoma migration. a A migration assay was performed in the MG63 cells with different migratory abilities (M10, M20, and M30). b MCP-1 protein production was detected in the MG63 cells with different migratory abilities (M10, M20, and M30) through Western blotting. c MCP-1 mRNA expression was compared between the MG63 cells with different migratory abilities (M10, M20, and M30) through a qPCR assay. d The cell migration ability of the osteoblast cell line hFOB 1.19 and the osteosarcoma cell lines MG63, U2OS and HOS was assessed using the Transwell assay. e-f Total mRNA and protein were collected from the indicated cell lines, and MCP-1 expression was detected using Western blotting and qPCR assay. g-h A migration assay and wound-scratching assay were performed, respectively, in the MG63, U-2OS, and HOS cells after stimulation with different concentrations of MCP-1 (1, 5, 10, and 50 ng/mL). i Representive image of wound-scratching assay in Fig. 1h. j A migration assay was performed in the MG63 cells in response to different concentrations of MCP-1 mAb (10 and 20 ng/mL). Results are expressed as mean ± SEM, n = 4. *p < 0.05 compared with MG63 (Fig. 1a-c), hFOB1.19 (Fig. 1d-f), control (Fig. 1g-h) and IgG (Fig. 1j), respectively