Fig. 4

Gene/miRNA expression in RAI-refractory PTCs. Principal component analysis (PCA) using the top most variable genes according to inter-quartile range. Specific samples features are showed separately: a Tissue type; b RAI class; c BRAF−/RAS-like subtype; d Driving lesion by PTC-MA; class Other includes 3 samples with gene fusions and 1 with pTERT mutation. Normal thyroid group is highlighted by a circle. Unsupervised hierarchical clustering of the top most variable genes according to inter-quartile range; Euclidean distance and Ward linkage. The following samples features are showed: e Tissue type. f RAI uptake and class. g Collection before and after RAI. h Driving lesion by PTC-MA. i BRAF−/RAS-like subtype. l Significantly deregulated miRNA, expression row Z-score; for 2 NT samples, depicted in white, the corresponding miRNA profiles were not available. m Thyroid differentiation (TD) score calculate as mean expression across 16 thyroid function related genes derived from TCGA. Abbreviations: RAI, radioiodine; LNM, lymph node metastasis; synLNM, synchronous lymph node metastasis; RAI+/D+, RAI uptake at the metastatic site and disease persistence; RAI−/D+, no RAI uptake at the metastatic site and disease persistence