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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Circular RNA MCTP2 inhibits cisplatin resistance in gastric cancer by miR-99a-5p-mediated induction of MTMR3 expression

Fig. 1

CircMCTP2 expression is downregulated in CDDP-resistant GC tissues and cells. a Heatmap of circular RNAs downregulated in BGC823CDDP and SGC7901CDDP cells compared to CDDP-sensitive GC cells. b qRT-PCR-based identification of circular RNAs that were decreased in CDDP-resistant GC tissues. c Expression of circMCTP2 in CDDP-resistant and CDDP-sensitive GC cells by qRT-PCR. d Confirmation of head-to-tail splicing of circMCTP2 with Sanger sequencing. e RT-PCR-based detection of back-spliced and linear MCTP2 in cDNA with or without RNase R and genomic DNA (gDNA). f CircMCTP2 and MCTP2 mRNA were treated with RNase R to detect their stability. g Results of actinomycin D assay. h FISH-based validation of preferential localization of circMCTP2 to the GC cell cytoplasm. i qRT-PCR-based confirmation of circMCTP2 localization mainly within the GC cell cytoplasm. (*p < 0.05, **p < 0.01. Data are expressed as the means ± SDs)

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