Fig. 3

CircMCTP2 represses autophagy in CDDP-resistant GC cells. a Expression of p62 protein was lower in CDDP-resistant GC patients than in CDDP-sensitive GC patients. b Expression levels of p62 were detected in GC tissues by western blotting. c-f GFP/mRFP-LC3 dots were observed and counted by confocal microscopy. g Protein levels of Beclin1, p62, and LC3-II were examined by western blotting. h and i The autophagic microstructure of CDDP-resistant GC cells was observed by transmission electron microscopy. CDDP treatment: 12 μM for 48 h in BGC823CDDP cells and 6 μM for 48 h in SGC7901CDDP cells. (*p < 0.05, **p < 0.01. Data are expressed as the means ± SDs)