Fig. 4

CircMCTP2 serves as a miRNA sponge of miR-99a-5p. a Candidate miRNAs predicted to be the potential binding targets of circMCTP2. b qRT-PCR analysis to examine the expression of circMCTP2 in BGC823CDDP and SGC7901CDDP cell lysates. c and d qRT-PCR analysis to examine the expression of candidate miRNAs after pull-down assays. e Analysis of luciferase activity in BGC823CDDP and SGC7901CDDP cells transfected with miR-NC or miR-99a-5p mimics and WT or mutant circMCTP2. f CircMCTP2 and miR-99a-5p localization to the cytoplasm of CDDP-resistant GC cells by FISH assay. g MiR-99a-5p upregulation in CDDP-resistant GC tissues by qRT-PCR. h MiR-99a-5p upregulation in CDDP-resistant GC cells by qRT-PCR. i Lack of effect of miR-99a-5p overexpression or knockdown on the expression of circMCTP2. (*p < 0.05, **p < 0.01. Data are expressed as the means ± SDs)