Fig. 6

SIRT6 stimulates the DNA damage repair pathway in doxorubicin-induced DNA damage. a U2OS and KHOS/NP cells transfected with control shRNA, shRNA for SIRT6, empty vector, or wild type-SIRT6 were treated with DMSO or 0.1 µM doxorubicin, and western blotting performed for SIRT6, ATM, phosphorylated ATM (p-ATM), Chk-2, phosphorylated Chk2 (p-Chk2), P53, phosphorylated P53 [p-P53(ser15)], H2AX, γH2AX, and GAPDH. Densitometry analysis of western blots was performed in triplicate and measured by using ImageJ software. b Whole cell lysates of U2OS and KHOS/NP treated with DMSO (0, control) or 0.1 µM doxorubicin were subjected to immunoprecipitation (IP) with an anti-SIRT6 antibody, anti-p-ATM antibody, or an isotype IgG (control) followed by immunoblotting (IB) with antibodies for SIRT6 and p-ATM. Input bands were obtained by performing western blot for SIRT6, p-ATM, and GAPDH. *; P < 0.05, **; P < 0.001