Fig. 1From: Loss of exosomal miR-146a-5p from cancer-associated fibroblasts after androgen deprivation therapy contributes to prostate cancer metastasisCharacteristics of CAFs derived from PCa patients and isolation of exosomes. a Immunofluorescence staining for α-SMA and FAP of primary CAFs and hTERT PF179T CAF (scale bars = 25 μm). b Exosomes derived from CAFs were analysed by transmission electron microscopy (TEM) (scale bars = 25 nm). c The expression of exosomal positive markers CD81 and TSG101 in CAFs and CAFs-derived exosomes were evaluated by western blot. d Nanoparticle Tracking Analysis showing the size and concentration of exosomes from ETOH-treated CAFs or DHT-treated CAFs. Data are shown as mean ± SD representing triplicate measurements. (Student’s t-test, *P < .05, **P < .01)Back to article page