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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Loss of exosomal miR-146a-5p from cancer-associated fibroblasts after androgen deprivation therapy contributes to prostate cancer metastasis

Fig. 3

Exosomes from CAFs after ADT promote EMT, migration and invasion of PCa cells in vivo. a Representative bioluminescence images of BALB/c nude mice that were injected with DU145 luciferase cells through tail vein and treated with either CAFs-derived exosomes or PBS via tail vein injections every other day for 2 weeks. Prior to tail vein injection, DU145 luciferase cells were pre-incubated with either CAFs-derived exosomes or PBS twice a day for 4 days. Images were taken in week 4 and week 8 (n = 5). b Tumour foci in lungs of mice after injecting the indicated DU145 cells and exosomes/PBS through tail vein. The right graph shows the number of lung metastases per mouse. c Immunohistochemical staining of E-cadherin and Vimentin in the lung metastases of ETOH/DHT-treated CAF Exo group and the control group (scale bars = 12.5 μm). d Average optical density of immunostaining of E-cadherin and Vimentin in the lung metastases of ETOH/DHT-treated CAF Exo group and the control group. Data are shown as mean ± SD representing triplicate measurements. (Student’s t-test, * P < .05, **P < .01, ***P < .001, ****P < .0001)

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