Fig. 6

MiR-146a-5p impairs cell migration and invasion by inhibiting EMT in PCa cells in vitro. a and b The effect of miR-146a-5p on the migration and invasion of LNcaP cells was assessed by a Transwell assay (scale bars = 25 μm). c and d The effect of miR-146a-5p on the migration and invasion of DU145 cells was assessed by a Transwell assay (scale bars = 25 μm). e and f The effect of miR-146a-5p on the RNA levels of EMT markers and MMPs in LNcaP and DU145 cells. g and h The effect of miR-146a-5p on the protein levels of EMT markers and MMPs in LNcaP were determined by western blot and densitometric quantification. i and j The effect of miR-146a-5p on the protein levels of EMT markers and MMPs in DU145 were determined by western blot and densitometric quantification. Data are shown as mean ± SD representing triplicate measurements. (Student’s t-test, ***P < .001, ****P < .0001)