Fig. 6

Suppression of migration, invasion and tube formation of endothelial cells by LAT1 knockdown and inhibition. a Wound healing assay using HUVECs transfected with control (NC) or LAT1-targeting siRNAs (LAT1 siRNA #1–3). b Quantification of migrated cell numbers in assays using LAT1 KD cells (left) and cells treated with JPH203 (0, 1, 10, 50 μM; right). c Invasion assay using LAT1 KD cells. d Quantification of covered surface areas in assays using LAT1 KD cells (left) and JPH203-treated cells (right). e Tube formation assay using LAT1 KD cells. f Quantification of total branching length of tubular network in assays using LAT1 KD cells (left), and JPH203-treated cells (right). b, d, n = 6; f, n = 7/8/8/4 for LAT1 KD, n = 8 for JPH203