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Table 1 Enzymes and metabolites in glycolysis for the detection of PDAC

From: Glycometabolic rearrangements--aerobic glycolysis in pancreatic cancer: causes, characteristics and clinical applications

Molecules Sources Levels Refs Detection methods
Enzymes
 HK-2 Gene profiles of PDAC patients (n = 143) up [179] Western blot [180], immunochemical staining [181], MS [182]
 PFK-p GeneChip hybridization of paired normal and tumor specimens from PDAC patients (n = 36) up [183] Immunohistochemistry [184], capillary electrophoresis [185]
 PKM-2 Tissue microarray of PDAC patients (n = 90) and the Oncomine database up [186] LC-MS/MS [187], western blot [188], immunochemical analysis [189]
 LDH-A Gene profiles of PDAC patients (n = 143) up [179] Immunohistochemistry [188], serum quantification [190]
 Aldolase Tissue microarray containing paired cancer and normal tissue specimens from PDAC patients (n = 96) up [137] Immunohistochemistry [191], western blot [137]
 GAPDH 2-D gel electrophoresis of paired cancer and normal tissue specimens from PDAC patients (n = 10) up [192] Immunoblotting [142], western blot [193], northern blot [194]
 PGK-1 2-D gel electrophoresis of paired cancer and normal tissue specimens from PDAC patients (n = 63) up [145] Protein microarray of serum [195], western blot [148]
 PGAM Immunohistochemistry of paired cancer and normal tissue specimens from PDAC patients (n = 54) up [153] Western blot [37], 2-D immunoblotting and LC-MS/MS [196]
 ENO-1 2-D gel electrophoresis of paired cancer and normal tissue specimens from PDAC patients (n = 10) up [192] Immunohistochemistry [37], western blot [197]
 PGI Microarray analysis of a human PDAC xenograft in a rat model up [198] Western blot and immunoprecipitation [199]
 TPI Gene profiles of PDAC patients (n = 143) up [179]
Metabolites
 Glucose Glycemic profiles of PDAC patients (n = 219) up [200] Blood draw for fasting blood glucose
 Lactate Pancreatic juice from PDAC patients (n = 79) and non-PDAC patients (n = 27) up [201] ERCP for pancreatic juice analysis
Others
 GLUTs Gene profiles of PDAC patients (n = 143) up [179] Immunohistochemistry
  1. TPI catalyzes the reaction of glycolysis in a non-KRAS-dependent manner and shows no correlation with PDAC progression