Fig. 1

Roquin1 expression was reduced in breast cancer patients and was associated with poor survival. a Roquin1 mRNA expression among normal (0) (n = 4), breast carcinoma (1) (n = 4), ductal breast carcinoma in situ (2) (n = 1), invasive ductal breast carcinoma (3) (n = 18), invasive lobular breast carcinoma (4) (n = 3), invasive mixed breast carcinoma (5) (n = 3), and male breast carcinoma (6) (n = 1). b Roquin1 expression was measured by qPCR in human breast tumor specimens (n = 19) compared with surrounding “normal” breast tissue (n = 19). c Roquin1 protein level was measured in human breast tumor tissues and normal mammary gland tissues. d-e Roquin1 protein (d) and mRNA (e) levels were measured by Western blot analysis and qPCR in human breast tumor cell lines and human normal mammary gland epithelial cell lines, respectively. f-g Kaplan–Meier overall survival (f) and relapse-free survival (g) curves of patients with breast tumors having low and high tumor Roquin1 transcripts. h–k Kaplan–Meier relapse-free survival curves of patients with luminal A (h), luminal B (i), basal (j), and Her2+ (k) breast tumor having low and high tumor Roquin1 transcripts