Fig. 2

Roquin1 inhibits cell proliferation and induced G1/S phase cell cycle arrest in breast tumor cells. a Roquin1/GFP fusion protein was detected by immunoblotting with anti-GFP and anti-Roquin1 antibodies, respectively. b-c Cell counting were conducted to measure the growth of MDA-MB-468 (b) and MCF7 (c) cells after the overexpression of Roquin1/GFP (n = 3). ***P < 0.0001. d-e MTT assay was performed to measure the cell activity of MDA-MB-468 (d) and MCF7 (e) cells overexpressing Roquin1/GFP protein (n = 3). *P < 0.05. f-g Cell cycle was analyzed by FCM in MDA-MB-468 (f) and MCF7 (g) cells after Roquin1 overexpression. The proportions of cells in the G1, G2, and S phases are shown (n = 3). *P < 0.05. h p21 protein was detected by immunoblotting with an anti-p21 antibody at different time points after Roquin1 overexpression in MDA-MB-468 and MCF-7 cells. i Apoptosis indicators PARP-1 and caspase-3 were measured by immunoblotting at different time points after Roquin1 overexpression in MDA-MB-468 and MCF-7 cells. j Flow cytometric analyses of apoptotic cells in MDA-MB-468 and MCF7 cells with Roquin1 overexpression compared with their control cells