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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Roquin1 inhibits the proliferation of breast cancer cells by inducing G1/S cell cycle arrest via selectively destabilizing the mRNAs of cell cycle–promoting genes

Fig. 5

Roquin1 knockdown stabilized cell cycle–promoting gene transcripts and promoted G1/S cell cycle progression. a Roquin1 knockdown was validated by western blot analysis. b-c Cell proliferation was measured by cell counting (b) and MTT assay (c) in MDA-MB-231 cells after knocking down Roquin1 at the indicated time points (n = 3). *P < 0.05. d Cell cycle–promoting gene mRNAs were measured in the Roquin1 knocked down MDA-MB-231 cells by qPCR. **P < 0.01 between two groups. e–h The half-lives of cell cycle–promoting genes were measured in Roquin1 knockdown MDA-MB-231 cells. i Cell cycle analysis was performed in MDA-MB-231 cells after the knockdown of Roquin1. The proportions of cells in the G1, G2, and S phases are shown (n = 3). **P < 0.01. j MDA-MB-231/Roquin1-knockdown cells were infected with scramble/lentivirus or shRNA lentivirus targeting CCNE1 and MCM2, respectively. Total RNA was extracted to measure the levels of mRNAs of CCNE1 and MCM2. *P < 0.05. k MTT assay was conducted to measure the cell growth of MDA-MB-231 cells after the co-knockdown of Roquin1 and CCNE1/MCM2. l Cell cycle analysis was performed in MDA-MB-231 cells after the co-knockdown of Roquin1 and CCNE1/MCM2. **P < 0.01

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