Fig. 7
Roquin1 suppressed breast tumor growth and metastasis. a Tumor growth curves in nude mice received 5 × 106 MDA-MB-468/Roquin-GFP and MDA-MB-468/GFP cells (n = 6/group). ***P < 0.0001 between two groups. b MDA-MB-468/GFP and MDA-MB-468/Roquin1/GFP tumors were collected and compared at the end of the experiment. c H&E staining of lung tissue sections from nude mice bearing MDA-MB-468/GFP or MDA-MB-468/Roquin1–GFP tumors. Scale bar, 50 μm. d Experimental flow chart for tumor treatment with Roquin1-expressing adenovirus in vivo. MDA-MB-231 cells (3 × 106 cells/100 μL of PBS) were injected into nude mice to establish a tumor mass. Tumors were treated with adenovirus (109 PFU adenovirus in 100 μL of PBS) every other day when tumors grew to a certain size (~ 5 mm in diameter). e Tumor growth curves in nude mice after treatment with adenovirus. Black arrows indicate the time point of adenovirus injection. f Quantification of the number of metastatic foci of each mouse treated with control or Roquin1-expressing adenovirus. g Total protein was extracted from tumor tissues to detect the expression of Roquin1/GFP, CCNE1, and MCM2 by immunoblotting with anti-GFP, anti-CCNE1, and anti-MCM2 antibodies, respectively. β-actin was used as a loading control. h-i Pearson’s Correlation analysis between Roquin1 and CCNE1 (h) and MCM2 (i) expression levels in log2 values in human breast cancer patients (n = 1006). j-k Kaplan–Meier relapse-free survival curves of patients with breast cancer having low and high levels of tumor CCNE1 (j) and MCM2 (k) transcripts