Fig. 2

GOLPH3L promotes tumorigenesis of breast cancer cells. a The proliferation of T47D cells before and after GOLPH3L alteration. The cell number was determined with EdU-high content screening in the cellular proliferation assay; n = 3, * p < 0.05, ** p < 0.01. b Knockdown and overexpression of GOLPH3L in T47D cells affected apoptosis. The cells were counted with a fluorescein assay; n = 3, * p < 0.05, ** p < 0.01. c Altering the expression of GOLPH3L inhibited the migration of T47D cells using a Transwell assay; n = 3, * p < 0.05, ** p < 0.01. d The knockdown of GOLPH3L significantly blocked the cell cycle at the G0/G1 phase according to the fluorescein assay at OD 405 nm; n = 3, * p < 0.05. e Analysis of the protein level of GOLPH3L in T47D cells before and after silencing (top panel). Suppressing the expression of GOLPH3L inhibited the tumor growth of T47D cells in nude mice, n = 5, ** p < 0.01 (three bottom panels)