Fig. 4

GOLPH3L promotes glucose metabolism in breast cancer cells. a Heat map of significantly altered metabolites after GOLPH3L suppression in the positive ion mode (left panel) and negative ion mode (right panel); p-values< 0.05 are indicated. b Bubble diagram of KEGG pathways related to the inhibition of GOLPH3L (left panel) and GO analysis of pathways affected by GOLPH3L silencing (right panel); p-values< 0.05 are indicated. c Correlation between different metabolites altered by the expression of GOLPH3L in the positive ion mode (top panel) and negative ion mode (bottom panel); p-values< 0.05 are indicated. d and e ECAR in cells of the control groups and GOLPH3L-knockdown group transfected with empty vector or siRNA in response to glucose, oligomycin and 2-DG. Data are presented as the mean value ± SD, two-way ANOVA with Tukey’s multiple comparison test, n = 3. Independent experiments for each group, p-values< 0.05 were indicated