Fig. 5

GOLPH3L promotes the expression of SERPINE1, which regulates tumorigenic activities in breast cancer cells. a Heat map of transcriptome alterations after silencing GOLPH3L expression in T47D cells; p-values are indicated. b Bubble diagram of KEGG pathways (left panel) and GO analysis of pathways (right panel) related to transcriptome alteration after the inhibition of GOLPH3L; p-values< 0.05 are indicated. c The mRNA expression of SERPINE1 was decreased by GOLPH3L silencing, and p-values< 0.01 are indicated. d Protein-protein interactions of differentially expressed mRNA of proteins. e The protein levels of SERPINE1 were higher in breast cancer tissue samples than in adjacent normal tissues (top panel), and the protein levels of GOLPH3L were higher in breast cancer cell lines than in normal mammary epithelial cells (bottom panel). f The expression of GOLPH3L was correlated with SERPINE1 expression, and SERPINE1 overexpression reversed the suppression of SERPINE1 caused by GOLPH3L knockdown. g SERPINE1 promoted the proliferation and survival of breast cancer cell lines; n = 3, * p < 0.05, ** p < 0.01