Fig. 5

Hypoxia-induced sorafenib resistance and mitophagy are partially mediated by miR-210-5P. a Electron micrographs showing mitophagy in hypoxia-cultured Huh7 cells transfected with miR-210-5P mimic or inhibitor treated cells with or without CoCl₂. b Immunoblot showing expression levels of indicated proteins in miR-210-5P mimic or inhibitor treated cells with or without CoCl₂. c, d Mito-Tracker fluorescence intensities of the indicated huh7 (c) and LM3 (d) cells. e Immunoblot showing expression levels of indicated proteins in Huh7 cells treated with miR-210-5P inhibitor and 1% O₂ with/without chloroquine. f-h Representative FACS analyses of apoptosis rates in Huh7 cells transfected with miR-210-5P mimic or inhibitor with or without hypoxia condition under sorafenib treatment. i Immunoblot showing expression levels of indicated proteins in miR-210-5P mimic or CoCl₂ and miR-210-5P inhibitor-treated Huh7 and LM3 cells under sorafenib treatment. Each bar represents the mean ± SEM of three independent experiments. *P < 0.05 and ** P < 0.01