Fig. 2

CircSOD2 promoter is intensively modified by H3K27ac and H3K4me3. a H3K27ac and H3K4me3 ChIP-seq results from HEPG2. circSOD2 and SOD2 promoter region were labeled with red, chromosome region spanning circSOD2 transcripts was labeled with yellow. H3K27ac (b) and H3K4me3 (c) ChIP-qPCR signal on circSOD2 promoter and control regions from HCC tumor tissues and adjacent normal liver tissues, IgG was used as control. Enrichment of EP300 (d) and WDR5 (e) on circSOD2 promoter and control regions from HCC tumor tissues and adjacent normal liver tissues. f Western blot results of EP300 after CRISPR-CAS9 mediated gene depletion, GAPDH was used as control. g H3K27ac signal on circSOD2 promoter after deleting EP300. h circSOD2 expression after deleting EP300. i Western blot results of WDR5 after CRISPR-CAS9 mediated gene depletion, GAPDH was used as control. j H3K4me3 signal on circSOD2 promoter after deleting WDR5. k circSOD2 expression after deleting WDR5. *P < 0.05, **P < 0.01, ***P < 0.001