Fig. 2

The influences of ZNF703 on proliferation and apoptosis and cell cycle in ovarian cancer cells. a Overexpression of ZNF703 promoted cell proliferation of ovarian cancer cells in MTT assay in OVCAR3 and ES-2 cell lines. b ZNF703-siRNA inhibited cell proliferation of ovarian cancer cells in MTT assay in CAOV3 and SKOV3 cell lines. c ZNF703 Overexpression decreased the cell apoptosis in OVCAR3 and ES-2 cell lines. d ZNF703-siRNA increased apoptosis of CAOV3 and SKOV3 cell lines. e Ovarian cells passed into S and G2/M phases after ZNF703 Overexpression. f G0/G1 phase arrest of ovarian cancer cells after ZNF703 siRNA transfection. g The increased proteins levels of PCNA, Bcl2/Bax, cyclin D1 were detected by western blot after overexpressed ZNF703. h Western blot analysis showed that the expression levels of PCNA, Bcl2/Bax, cyclin D1 proteins in ovarian cancer cells decreased after transfection with ZNF703-siRNA. The experiment was repeated three times. For western blot, GAPDH was used as an internal control. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001