Fig. 4

ZNF703 interacted with HE4 and HE4 promoted the nuclear translocation of the ZNF703. a The co-immunoprecipitation analysis of ZNF703 and HE4 was performed in ovarian cancer cells (The left two were performed in CAOV3 cell line and the right two were performed in OVCAR3 cell line. The corresponding molecular weight was added to the right side). b The immunofluorescence co-localization of ZNF703 and HE4 was performed in CAOV3 and OVCAR3 cells (The green color was for ZNF703 and the red color was for HE4, DAPI was blue, the colocalization was yellow) (×800). The arrow indicated the co-localization in the nucleus. c, d Western blot analysis of intracellular total ZNF703 protein in CAOV3 and OVCAR3 cells transfected with the overexpressed HE4 plasmid or control, or HE4 siRNA or control. e, f Western blot determined nuclear and cytoplasmic distribution of ZNF703 after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with the overexpressed HE4 plasmid or control. g, h Nuclear and cytoplasmic distribution of ZNF703 tested by western blot after nuclear cytoplasmic fractionation in CAOV3 and OVCAR3 cells transfected with HE4 siRNA or control. i, Immunofluorescent staining of ZNF703 in CAOV3 and OVCAR3 cells transfected with HE4 siRNA or control (× 800). The statistical results are on the right side of their corresponding positions. The experiment was repeated three times. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001