Fig. 6

ZNF703 promoted the PEA15 expression epigenetically and phosphorylation at Ser116. a, b ZNF703 mRNA expression in the various tumors from GEPAI database and Oncomine database. c, d Validation of PEA15 mRNA levels changes using qRT-PCR analysis after upregulated or downregulated ZNF703 expression in cell lines (2^-ΔΔCт.). e The PEA15 protein and phosphorylation levels changes in cell lines transfected with the overexpressed ZNF703 or control, or ZNF703 siRNA or control. f The relative gray value calculated for Fig. 6e. g Schematic diagram of dual luciferase reporter gene construction. h Dual luciferase activity assays were performed using firefly luciferase reporter vectors and Renilla luciferase served as an internal control. The experiment was repeated three times. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001