Fig. 3

Depletion of VANGL1 sensitized LUAD cells to irradiation. VANGL1-shRNA and the overexpression vectors were transfected into LUAD cells to knock down or overexpress VANGL1. LUAD cells with VANGL1 knockdown further underwent 2 Gy irradiation. a PCR was performed to measured VANGL1 expression in A549 and H1299 cells. Cell viability (b), apoptosis rate (c) and invasion capacity (d) were also evaluated after the transfection and irradiation treatments. e IF was performed to determine the level of γH2AX in A549 and H1299 cells. VANGL1 overexpression alone had no effect on γH2AX level (data not shown). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control group. Unpaired two-tailed t-tests (n = 3)