Table 1 The primers for PCR assay
Gene name | Direction | Sequence |
|---|---|---|
VANGL1 | forward | 5'-ATGTCACAGTCCGTGTTCCA-3' |
reverse | 5'-CCAGAAGTGCCGAATCATTT-3' | |
METTL3 | forward | 5'-TTGTCTCCAACCTTCCGTAGT-3' |
reverse | 5'-CCAGATCAGAGAGGTGGTGTAG-3' | |
IGF2BP2 | forward | 5'-AGCTAAGCGGGCATCAGTTTG-3' |
reverse | 5'-CCGCAGCGGGAAATCAATCT-3' | |
IGF2BP3 | forward | 5'-ACGAAATATCCCGCCTCATTTAC-3' |
reverse | 5'-GCAGTTTCCGAGTCAGTGTTCA-3' | |
beta-actin | forward | 5'-CATGTACGTTGCTATCCAGGC-3' |
reverse | 5'-CTCCTTAATGTCACGCACGAT-3' | |
miR-29b-3p | forward | 5'-ACACTCCAGCTGGGTAGCACCATTTGAAATC-3' |
reverse | 5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTG AGAACACTGA-3' | |
miR-15b-5p | forward | 5’-TAGCAGCACATCATGGTTTACA-3’ |
reverse | 5’-TGCGTGTCGTGGAGTC-3’ | |
miR-200a-3p | forward | 5ʹ-TAACACTGTCTGGTAACGATGT-3’ |
reverse | 5ʹ-CATCTTACCGGACAGTGCTGGA-3’ | |
U6 snRNA | forward | 5'-CTCGCTTCGGCAGCACA-3' |
reverse | 5'-AACGCTTCACGAATTTGCGT-3' |