Fig. 1

Histone peptide modification states in pediatric glioma cell lines. a Unsupervised hierarchical clustering of pediatric glioma cell line histone tail modification profiles reveals clustering by H3K27M mutation status. b-e Bar graph showing that relative abundance of modifications at H3.1K27/K36 and H3.3K27/K36 in glioma cell lines are distinct by H3.3K27M mutation status. f-g Statistically significant differences in H3K79me2 and H4K16ac abundance are observed in H3.3K27M mutant glioma cell lines (n = 3) compared to H3.3K27 WT (n = 4). * p < 0.05 compared to H3.3K27 WT group, independent-sample t test, two-tailed. un = unmodified, me1 = mono-methylation, me2 = di-methylation, me3 = tri-methylation, ac = acetylation