Fig. 2

Tissue histone peptide modification states differ by H3.3K27M mutation status. a Unsupervised hierarchical clustering of glioma and normal brain tissue histone tail modification profiles reveals clustering by H3.3K27M mutation status. b-e Bar graph showing relative modifications at H3.1K27/K36 and H3.3K27/K36 in tissue specimens are distinct by H3.3K27M mutation status. f Statistically significant modifications of H2 and H4 acetylation are observed in H3.3K27M mutant tissues (n = 9) compared to H3.3K27 WT (n = 3).* p < 0.05 compared to H3.3K27 WT group, independent-sample t test, two-tailed. un = unmodification, me1 = mono-methylation, me2 = di-methylation, me3 = tri-methylation, ac = acetylation