Fig. 5

WNT4 signaling in fibroblasts. a β-catenin translocation from the cytoplasm to the nucleus in the NF#1, NF#2 treated with WNT4 protein (400 ng/ml, 24 h). Scale bar, 50 μm. b and c ICG-001 completely blocked elevation of α-SMA and fibronectin at 10 μM was detected by western blot. Scale bar, 20 μm. d and e The effect of WNT4 on the formation of heterospheroids and gel contraction ability was completely inhibited by ICG-001 (10 μM). Scale bar, 20 μm. Measurement data were presented as the mean ± SD. *P < 0.05; NS, no significance