Fig. 7
WNT4 levels can be regulated by miR-497. a Western blotting assays and RT-qPCR showed the protein and mRNA expression of WNT4 in CRC cells after transfection with miR-497 mimics. b The target sites of miR-497 in the 3′-UTR of WNT4 shown as a schematic representation. c Wild-type or mutant 3′-UTR constructs of WNT4 were cloned into pMIR-REPORT™ vectors, respectively, and co-transfected with miR-497 mimics in LoVo cells. d and e The mRNA level of miR-497 and WNT4 in the paired colorectal cancer tissues and adjacent tissues. f An inverse correlation was found between miR-497 expression and WNT4 in 10 paired CRC tissues and adjacent normal tissues (Spearman’s correlation, P < 0.01, R2 = 0.7117)