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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: KLF7: a new candidate biomarker and therapeutic target for high-grade serous ovarian cancer

Fig. 2

KLF7 silencing inhibits proliferation of high-grade serous ovarian cancer (HGSOC) cells. a Representative western blot and densitometric analysis of KLF7 protein expression in a panel of HGSOC cell lines. GAPDH was used as loading control. b Relative KLF7 mRNA expression assessed by RT-qPCR analysis in OV-90 and PEO1 cells transiently transfected with specific siRNAs (siKLF7) and non-targeting siRNA pool as control (siC). Data are presented as fold change, calculated with the ΔΔCt method, using siC as reference sample. c Representative western blot and densitometric analysis of KLF7 protein expression in OV-90 and PEO1 cells transiently transfected with siKLF7 and with siC, as control. Cells were harvested 72 h post transfection and cell lysates were subjected to western blot with the specific antibody. GAPDH was used as loading control. d Bar chart representing proliferation assay for transfected OV-90 and PEO1 cells compared to respective control cells. Viable cells were counted at 24 h, 48 and 72 h from transfection. The mean cell proliferation at Time x (Tx) was expressed as average percentage increase relative to T = 0 h (T0). For all experiments, bars and error bars refer to mean and SD (Standard Deviation) of three experiments. N.d. = not detectable. To establish statistically significant differences, unpaired t-test was carried out. *p < 0.05; **p < 0.01

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