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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Rhenium N-heterocyclic carbene complexes block growth of aggressive cancers by inhibiting FGFR- and SRC-mediated signalling

Fig. 5

Effect of JVG045 on pancreatic cancer cell lines depends on mutationally activated KRAS. a Graph showing that in the BxPC-3 human pancreatic cancer cell line (which lacks a KRAS mutation) increasing doses of JVG045 had no significant effect on cell number. In contrast, SW1990, a pancreatic cancer cell line bearing a KRAS mutation and wt P53, showed enhanced sensitivity to increasing concentrations of JVG045. b Representative western blot showing the effect of JVG045 and ps27 (both at 10 μM) on the phosphorylation of FGFR1(Y653/654) and Src(Y416) in the presence or absence of the FGF ligand (20 ng/ml). ReCl was used as a negative control. c Effect of JVG045 (10 μM) in significantly reducing cell numbers in chemoresistant AsPC-1 tumorspheres enriched in cancer stem cells. d JVG045 (10 μM) shows no significant effect in KRAS wt BxPC-3 tumorspheres. e Effect of JVG045 (10 μM) in significantly reducing cell number in chemoresistant primary KPC tumorspheres enriched in cancer stem cells. Results are expressed as Mean ± SEM and are representative of at least three independent experiments

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