Fig. 2

Circ-LRIG3 enhances HCC aggressive phenotype. a, b The diagrammatic sketch showing the circ-LRIG3-overexpressing pLC5-ciR vector, the overexpression efficiency was verified by qRT-PCR. c, d The designed ASO against circ-LRIG3 junction site inhibiting circ-LRIG3 expression, the knockdown efficiency was verified by qRT-PCR. e-i CCK-8, EdU, Transwell migration and invasion assays in circ-LRIG3-overexpressing HepG2 and circ-LRIG3-silenced SMMC-7721 cells. j Circ-LRIG3-overexpressing HepG2 and circ-LRIG3-silenced SMMC-7721 cells were stained with PE and 7-ADD dyes, followed by flow cytometry analysis. **P < 0.01,***P < 0.001