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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling

Fig. 3

Circ-LRIG3 activates STAT3 signaling in HCC cells. a, b mRNA sequencing in circ-LRIG3-silenced SMMC-7721 cells, followed by GSEA analysis the enrichment of STAT3 pathway. c, d qRT-PCR analysis of the indicated gene expression in circ-LRIG3-overexpressing HepG2 and circ-LRIG3-silenced SMMC-7721 cells. e Luciferase reporter assay detecting the effect of circ-LRIG3 on the activity of STAT3 pathway. f Western blot analysis of p-STAT3 and STAT3 protein levels in circ-LRIG3-overexpressing HepG2 and circ-LRIG3-silenced SMMC-7721 cells, GAPDH was used as a loading control. g The correlation between circ-LRIG3 and p-STAT3 in 130 HCC tissues. h CCK-8 and Transwell assays detecting the cell viability and invasion in circ-LRIG3-overexpressing HepG2 cells treated with C188–9. *P < 0.05, **P < 0.01

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