Fig. 5

Fmo5 plays an important role in the AFLD-induced apoptosis and inflammatory response in the small intestine and liver tissues of mice and is closely related to PPARα expression and the NF-κB signaling pathway in vivo (a) Venn map of the one overlapping gene from the hub genes of the above 4 PPI network. b The heat map of Fmo5 expression in GSE8006 and GSE40334 datasets. c Western blot assay was used to detect the expression of Fmo5, PPARα, cleaved caspase-3, phospho-NF-κB p65, NF-κB p65, IL-6, IL-4, and TNF-α in the small intestine and liver tissues between the control group and the AFLD group. d ELISA assay was used to detect the levels of ALT, AST, and TG in blood sample between the control group and the AFLD group. e ELISA assay was used to detect ROS level in blood sample between the control group and the AFLD group. f ELISA assay was used to detect the levels of IL-6/− 4 and TNF-α in blood sample between the control group and the AFLD group. g H&E staining was used to observe the pathological change in liver tissues among the four group, Oil Red staining was used to observe the lipid deposition in liver tissues among the four group, and TUNEL was used to detect the apoptosis in liver tissues among the four group (magnification times: 100x and 200x; label with an arrow as to indicates a lipid deposit). h Western blot assay was used to detect the expression of Fmo5, PPARα, phospho-NF-κB p65 and NF-κB p65 in liver tissues among the four group. i IHC assay was detect the expression of PPARα, cleaved caspase-3, and phospho-NF-κB p65 in liver tissues among the four group (magnification times: 100x and 200x). GAPDH used as a load control. Data are presented as the mean ± standard deviation. ** P < 0.01 vs. Control group, ## P < 0.01 vs. AFLD group, and ^^ P < 0.01 vs. Fmo5−^/− group