Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Loss of miR-192-5p initiates a hyperglycolysis and stemness positive feedback in hepatocellular carcinoma

Fig. 4

c-Myc as well as two glycolytic enzymes were miR-192-5p targets. a Genes negatively correlated with miR-192-5p (r < − 0.3) and significantly altered in HCC192Low vs. HCC192High (Left panel) and predicted miR-192-5p binding sites in 3′-UTR or coding regions (CDS) of human PFKFB3, GLUT1, MYC and MCT4 (Right panel). b Western blotting of PFKFB3, GLUT1, c-Myc and MCT4 in HLE and HLF cells infected with lentivirus pmiR-Ctrl or pmiR-192. c Luciferase activities were measured using reporters with WT or MT miR-192-5p binding sites of three genes in the 3′-UTR or CDS of luciferase vector. 192pos reporter was used as the positive control. d ECAR was measured in HLF-WT and HLF-192KO cells transfected with 50 nM si-Ctrl, si-PFKFB3, si-GLUT1, and si-MYC respectively. e RT-PCR analysis of five CSC surface markers in HLF-WT and HLF-192KO cells infected with si-Ctrl, si-PFKFB3, si-GLUT1, as well as the combination of si-PFKFB3 and si-GLUT1. UGT2B7 was measured as a negative control. f Flow cytometry analysis of CD24+ and CD44+ populations in HLF-WT cells transfected with si-Ctrl, HLF-192KO cells transfected with si-Ctrl, and HLF-192KO cells transfected with the combined si-PFKFB3 and si-GLUT1. Student t-test was performed for (c.e.). *, p < 0.05

Back to article page