Fig. 6

Overproduced lactate from HCC cells with miR-192-5p loss activated ERK pathway in environmental non-tumor cells. a Western blotting analysis for pERK and total ERK in LX2, THP1 and HL7702 cells treated with 0, 10, 20 mM lactate. b Western blotting analysis for pERK and total ERK in LX2, THP1 and HL7702 cells upon co-culture with HLF-WT or -192KO cells infected with lentivirus pmiR-Ctrl or pmiR-192 in a co-culture chamber system. c RT-qPCR analysis of MCT1 and NDRG3 in different HCC and non-HCC cell lines. d Western blot analysis of pERK and total ERK in LX2 cells transfected with si-NDRG3 or si-MCT1 and treated with different doses of Lactate. e Western blot analysis of pERK and total ERK in THP1 cells transfected with si-NDRG3 or si-MCT1 and treated with different doses of lactate. Student t-test was performed. *, p < 0.05