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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion

Fig. 1

PGRN promotes M2 polarization of macrophages. a-b. Breast cancer PY8119 cells were injected in situ into the fat pads of C57 wild-type mice and PGRN knock out mice (n = 5 per group). a. Tumor volume curve. b. Survival curve of mice. c. F4/80, iNOS and CD206 expression were detected by IHC in breast cancer tissue sections of WT and PGRN KO mice respectively. d-e. RAW264.7 macrophage cell line was treated with PGRN recombinant protein and LPS or IL-4. d. iNOS and Arg1 expression were examined by western blot. e. M1 markers (IL-12, TNF-α) and M2 markers (Arg1, IL-10) were tested by PCR. (F-G) WT and PGRN KO mouse peritoneal macrophages were treated with LPS or IL-4. f. Western blot was performed to analyze iNOS, and Arg1 expression. g. The differences in the expression of IL-12, TNF-α and Arg1, and IL-10 were measured by PCR. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

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