Fig. 2

CircLMO7 acts as a sponge of miR-30a-3p. a. Venn diagram showing the target miRNAs of circLMO7 predicted by TargetScan7.2 and RegRNA2.0. b. Target miRNAs overlapping between TargetScan7.2 and RegRNA2.0. c. Pull-down efficiency of the circLMO7 probe. d After pull-down experiments, we found that the binding efficiency between circLMO7 and miR-30a-3p was the highest. e. Sequence diagram of the binding sites between circLMO7 and miR-30a-3p. f. Map of the mutations of the binding sites between circLMO7 and miR-30a-3p. g. A luciferase reporter assay demonstrated that circLMO7 and miR-30a-3p bound to each other. h. RNA fluorescence in situ hybridization confirmed the binding relationship between circLMO7 and miR-30a-3p; scale bar = 10 μm. i. qRT-PCR showed that the expression of miR-30a-3p in GC tissues was significantly lower than that in adjacent normal tissues. j. qRT-PCR showed that circLMO7 and miR-30a-3p levels were negatively correlated. All data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001