Fig. 3

Overexpression of circLMO7 or knockdown of miR-30a-3p promotes the proliferation, migration and invasion of GC cells. a, b. Colony formation and EdU assays showed that overexpression of circLMO7 or knockdown of miR-30a-3p promoted the proliferation of GC cells, while knockdown of circLMO7 or overexpression of miR-30a-3p inhibited this process; EdU scale bar = 25 μm. c. Transwell assays showed that overexpression of circLMO7 or knockdown of miR-30a-3p promoted the migration and invasion of GC cells, while knockdown of circLMO7 or overexpression of miR-30a-3p had the opposite effects; scale bar = 100 μm. d. Overexpression of circLMO7 or knockdown of miR-30a-3p promoted the growth of human GC organoids, while the growth of human GC organoids was inhibited after circLMO7 had been knocked down or miR-30a-3p had been overexpressed; scale bar = 20 μm. e. Overexpression of circLMO7 or knockdown of miR-30a-3p promoted the migration and invasion of GC cells through the EMT pathway, while knockdown of circLMO7 or overexpression of miR-30a-3p showed the opposite results. All data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001