Fig. 6

CircLMO7 promotes GC malignant biological functions through glutamine metabolism. a. TCGA database analysis showed that GLS was highly expressed in GC tissues. b. qRT-PCR verified that GLS was highly expressed in GC tissues. c, d. qRT-PCR and Western blot analysis showed that circLMO7 and GLS were positively related. e-h. Overexpressing circLMO7 increased the expression levels of GLN, GLS, GLU and α-KG. Knocking down circLMO7 blocked this phenomenon. i, j. Colony formation and EdU assays showed that the promoting effect of ov-circLMO7 on GC cell proliferation was rescued after cotransfection with si-GLS; EdU scale bar = 25 μm. k. Transwell assays showed that si-GLS rescued the promoting effect of ov-circLMO7 on GC cell migration and invasion; scale bar = 100 μm. l. The active oxygen assay showed that the inhibitory effect of ov-circLMO7 on the production of reactive oxygen species in GC cells was rescued after cotransfection with si-GLS; scale bar = 50 μm. All data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001